Technical Support - InBios International, Inc.

Can the plates be manually washed?

All validations of our ELISA kits are done using automatic plate washers. We do not formally recommend manually washing your plate. If you must manually wash your plate, we recommend using a multichannel pipette rather than a squirt bottle. This will provide more reproducible and controllable volume delivery. Plates should be washed with 300uL of 1X ELISA Wash Buffer, six times in a row. We recommend that you tap your plate out between rinses to remove as much excess Wash Buffer as possible. Additionally, because manual plate washing is more time consuming than using a plate washer, we do not recommend running multiple plates at once.

Can I use a plate sealer instead of parafilm?

Yes. Our main concern during incubations is fluid loss due to evaporation. As long as that is minimized by the plate sealer (or a similar cover), you should be fine to proceed.

Are your assays validated on any automated ELISA processing systems?

Unless noted in the product instructions for use, we have not formally validated our assays on any automated ELISA processing system. We do have customers who have successfully validated some of our assays on automated systems. Customization may be required. Consult with an InBios representative if you are considering automating an assay.

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Can the plates be manually washed?

Can I use a plate sealer instead of parafilm?

Are your assays validated on any automated ELISA processing systems?

No control line appears

The test line is very faint. How do I read the result?