Technical Support firstname.lastname@example.org
InBios Contact Info
InBios International, Inc.
307 Westlake Ave. N., Suite 300
Seattle, Washington 98109
Toll Free 1.866.462.4671
All validations of our ELISA kits are done using automatic plate washers. We do not formally recommend manually washing your plate. If you must manually wash your plate, we recommend using a multichannel pipette rather than a squirt bottle. This will provide more reproducible and controllable volume delivery. Plates should be washed with 300uL of 1X ELISA Wash Buffer, six times in a row. We recommend that you tap your plate out between rinses to remove as much excess Wash Buffer as possible. Additionally, because manual plate washing is more time consuming than using a plate washer, we do not recommend running multiple plates at once.
Yes. Our main concern during incubations is fluid loss due to evaporation. As long as that is minimized by the plate sealer (or a similar cover), you should be fine to proceed.
We have not formally validated our assays on any automated ELISA processing system. We do have customers who have successfully validated some of our assays on automated systems. Customization may be required. Consult with an InBios representative if you are considering automating an assay.
Rapid Tests FAQs
The test is invalid if no control line appears, regardless of whether a test line appears. Discard and retest. Retest using a new test strip and fresh serum.
Any line (including faint or weak lines) appearing in the test area should be considered positive.